Researchers at Cranfield University in the UK have published a paper in the journal Frontiers in Genome Editing, announcing the successful development of a new CRISPR-Cas9-based gene editing method for raspberries. This technology, achieved through DNA-free gene editing, provides a new solution for extending raspberry shelf life and improving crop sustainability.

The research team isolated protoplasts from raspberry microplant leaf tissue and used ribonucleoprotein (RNP) complex transfection to achieve precise gene editing. The method targets specific genes such as NPR1, whose similar editing in tomatoes has been shown to enhance resistance to gray mold (Botrytis cinerea). PhD student Ryan Creeth stated: "Precision breeding technologies are crucial for addressing food waste, improving food sustainability, and enhancing nutrition. We need to apply cutting-edge technologies such as DNA-free gene editing to a wider range of crop species."

Compared with traditional breeding methods, gene editing technology can shorten the variety improvement cycle from more than ten years to approximately 12 months. This technology does not introduce exogenous DNA, and the resulting genetic changes are difficult to distinguish from natural mutations, complying with the regulatory requirements of the UK's Genetic Technology (Precision Breeding) Act 2023. The researchers noted that the method can also be used to develop raspberry varieties with sweeter and larger fruit, seedless traits, or higher climate adaptability.

Currently, the research team is working to overcome the technical challenge of regenerating complete plants from edited protoplasts. Once successful regeneration is achieved, it will enable the stable production of gene-edited raspberries with characteristics such as mold resistance, providing a new path to reduce food waste and improve agricultural production efficiency.